Raw data for publication "Polyphenol Diversity and Chemotype Variation in Origanum majorana and Related Species: Implications for Chemotaxonomic Differentiation, Standardisation and Genotype Selection"

Raw data for the publication:

Polyphenol Diversity and Chemotype Variation in Origanum majorana and Related Species: Implications for Chemotaxonomic Differentiation, Standardisation and Genotype Selection

Brigitte Lukas, Johannes Novak, Magdalena Neumüller, Jennifer Romana Valek, Salme Ahmed, Zehra Aytaç, Ahmet Gümüşçü

Abstract

This study focuses on the qualitative and quantitative composition of non-volatile compounds in wild and commercial O. majorana, the three other species of section Majorana (O. dubium, O. syriacum and O. onites) as well as two more distantly related species, O. minutiflorum (section Chilocalyx) and O. vulgare (section Origanum). Methanolic extracts from 657 individual plants from 59 populations and seed accessions were analysed using high performance liquid chromatography (HPLC). The extracts of wild O. majorana predominantly consisted of arbutin (mean of 9 area percent), apigenin 6,8-di-glucopyranoside (4%), luteolin 7-glucuronide (9%), rosmarinic acid (19%), apigenin 7-glucuronide (7%), salvianolic acid B (8%), two unidentified flavonoids (4 and 7%, respectively) and blumeatin (6%). Differences in the proportions of these compounds enabled the differentiation between wild and commercial O. majorana plants by principal component analysis (PCA). The polyphenol spectrum of O. majorana was remarkable within the section Majorana. Origanum dubium, O. syriacum and O. onites displayed a markedly distinct polyphenol chemotype, with their clusters intersecting centrally between the well-defined clusters of O. majorana, O. minutiflorum and O. vulgare. When considering qualitative and quantitative extract composition of the economically significant Origanum species, O. majorana and O. vulgare are superior sources of phenolic acids while O. dubium, O. syriacum and O. onites are more abundant in flavonoid glycosides. There is high potential for the selection of genotypes exhibiting elevated levels of arbutin (O. majorana wildtype, up to 99 mg/g dry weight), apigenin 6,8-di-glucopyranoside (O. dubium, up to 118 mg/g), rosmarinic acid (commercial O. majorana, up to 53 mg/g; O. vulgare ssp. vulgare up to 62 mg/g) or salvianolic acid B (O. majorana wildtype, up to 37 mg/g).

用于发表论文的原始数据：《牛至属马郁兰及近缘物种的多酚多样性与化学型变异：对化学分类鉴别、标准化及基因型筛选的启示》
Brigitte Lukas, Johannes Novak, Magdalena Neumüller, Jennifer Romana Valek, Salme Ahmed, Zehra Aytaç, Ahmet Gümüşçü

摘要
本研究聚焦野生及商业化马郁兰（Origanum majorana，以下简称O. majorana）、牛至属马郁兰组其余3个物种（O. dubium、O. syriacum与O. onites），以及另外2个亲缘关系稍远的物种——小花牛至（Origanum minutiflorum，奇萼组）和普通牛至（Origanum vulgare，牛至组）的非挥发性组分的定性与定量组成。
本研究采用高效液相色谱（High Performance Liquid Chromatography，以下简称HPLC）对来自59个种群与种子种质的657株单株的甲醇提取物进行了分析。野生马郁兰的提取物主要由以下成分构成：熊果苷（arbutin，占面积百分比均值9%）、芹菜素6,8-二葡萄糖苷（4%）、木犀草素7-葡萄糖醛酸苷（9%）、迷迭香酸（19%）、芹菜素7-葡萄糖醛酸苷（7%）、丹酚酸B（8%）、两种未鉴定黄酮类化合物（分别占4%与7%）以及蒲桃素（6%）。通过主成分分析（Principal Component Analysis，以下简称PCA），可基于上述化合物的比例差异区分野生与商业化马郁兰植株。
在马郁兰组中，马郁兰的多酚谱特征显著。O. dubium、O. syriacum与O. onites呈现出显著不同的多酚化学型，其聚类群在边界清晰的马郁兰、小花牛至与普通牛至聚类群的中心区域相互交叉。若考量具有经济价值的牛至属物种的提取物定性与定量组成，马郁兰与普通牛至是酚酸类物质的优质来源，而O. dubium、O. syriacum与O. onites的黄酮苷类成分含量更为丰富。筛选高含量目标成分的基因型具有较高潜力：例如熊果苷（野生马郁兰，最高可达99 mg/g干重）、芹菜素6,8-二葡萄糖苷（O. dubium，最高可达118 mg/g）、迷迭香酸（商业化马郁兰最高53 mg/g；普通牛至原亚种最高62 mg/g）以及丹酚酸B（野生马郁兰最高37 mg/g）。