Inhibition of A549 cell viability by trypacidin.

A549 cells were exposed for 1 h and 2 h with 10 µM and 50 µM trypacidin, then the medium containing trypacidin was replaced by fresh medium and cell viability was measured 24 h later using MTT assay. Cells were also exposed to trypacidin for 24 h before the MTT assay.将A549细胞（A549 cells）分别以10 μM和50 μM的曲帕西丁（trypacidin）处理1小时及2小时，随后移除含曲帕西丁的培养基并更换为新鲜培养基，于24小时后采用MTT比色法检测细胞活力；此外另有一组实验中，细胞先与曲帕西丁共孵育24小时后再进行MTT检测。