Dataset_14-d interval ovum pick up

The dataset contains information obtained in a two-treatment (FWS vs. control) and two-period (1 vs. 2) crossover design study run in a commercial dairy herd from Argentina to test the hypothesis that follicular wave synchronization (FWS) before ovum pick-up (OPU) modifies follicle size and increases oocyte recovery and in vitro embryo development in beef cows and that short intersession interval OPU schemes, such as 14-day intersession interval, lead to potential carryover effects. The objectives were to evaluate: 1- the effect of FWS on follicle size, oocyte recovery, and in vitro embryo development in cows subjected to OPU sessions with an interval of 14 days, and 2- the probability of a carryover effect due to the short intersession interval used. The treatment had neither effect on follicle size nor the number of oocytes (P = 0.81 and 0.53, respectively). However, treatment tended to affect the total embryo number (P = 0.06) given that cows receiving FWS had twice the number of embryos as control cows. Finally, treatment did not affect the number of frozen embryos (P = 0.76). The period affected the number of good oocytes (P = 0.05). The period also tended to affect the number of viable oocytes given that it also increased in the second one (P = 0.07). Finally, the period tended to affect the total number of embryos given that it decreased in the second one (P = 0.10). Otherwise, treatment by period interaction (testing for carryover effect) affected the total number of follicles (P = 0.05) and, also, the number of viable oocytes (P = 0.02). It also tended to have effect on the number of < 6 mm follicles (P = 0.13), the total number of oocytes (P = 0.11), the number of good oocytes (P = 0.12), and the number of regular oocytes (P = 0.13).

本数据集源自阿根廷某商业牛群开展的一项双处理（卵泡波同步化（Follicular Wave Synchronization, FWS）vs. 对照组）、两周期（周期1 vs. 周期2）交叉设计研究，旨在验证两项假说：其一，卵母细胞采集前（Ovum Pick-Up, OPU）实施FWS可改变卵泡大小，提升肉牛的卵母细胞回收率与体外胚胎发育能力；其二，间隔时长较短的OPU方案（如14天采集间隔）可能存在残留效应。
本研究的目标为评估：1. 采用14天间隔的OPU采集方案时，FWS对肉牛卵泡大小、卵母细胞回收率及体外胚胎发育能力的影响；2. 采用短间隔OPU方案所引发的残留效应发生概率。
实验结果显示：处理对卵泡大小与卵母细胞数量均无显著影响（分别对应P=0.81与P=0.53）。然而，处理对总胚胎数存在边际显著影响（P=0.06）：接受FWS处理的肉牛其胚胎数量为对照组的两倍。最终，处理对冻存胚胎数量无显著影响（P=0.76）。
周期因素对优质卵母细胞数量存在显著影响（P=0.05）；同时对有活力卵母细胞数量存在边际显著影响：第二周期的有活力卵母细胞数量有所提升（P=0.07）。此外，周期因素对总胚胎数存在边际显著影响：第二周期的总胚胎数有所下降（P=0.10）。
另外，处理与周期的交互作用（用于检测残留效应）对总卵泡数存在显著影响（P=0.05），同时对有活力卵母细胞数量也存在显著影响（P=0.02）；其对直径小于6mm的卵泡数（P=0.13）、总卵母细胞数（P=0.11）、优质卵母细胞数（P=0.12）及常规卵母细胞数（P=0.13）均存在边际显著影响。