An integrative single-cell multiomics profiling of human pancreatic islets identifies T1D associated genes, regulatory regions, and single nucleotide polymorphism [ATAC-seq]

To investigate the mechanism of DLK1 and RASGRP1 knockout in inducing beta cell apoptosis, we performed chromatin access profiling analysis using data obtained from ATAC-seq of DLK1 WT vs DLK1-/- and RASGRP1 WT VS RASGRP1-/- hPSCs differentiated beta cells. Comparative chromatin accessibility profiling analysis of ATAC-seq data for DLK1 WT vs DLK1-/- and RASGRP1 WT VS RASGRP1-/- hPSCs differentiated beta cells.

为探究DLK1与RASGRP1基因敲除诱导β细胞凋亡的分子机制，本研究利用DLK1野生型（wild type，WT）与DLK1纯合敲除（DLK1-/-）、RASGRP1野生型（wild type，WT）与RASGRP1纯合敲除（RASGRP1-/-）人类多能干细胞（human pluripotent stem cells，hPSCs）分化所得β细胞的ATAC-seq（转座酶可及性染色质测序）数据，开展了染色质可及性谱分析。针对上述DLK1野生型（WT）vs DLK1-/-、RASGRP1野生型（WT）vs RASGRP1-/-的hPSCs分化β细胞的ATAC-seq数据，本研究完成了比较染色质可及性谱分析。