Data from: Large effect quantitative trait loci for salicinoid phenolic glycosides in Populus: Implications for gene discovery

Genomic studies have been used to identify genes underlying many important plant secondary metabolic pathways. However, genes for salicinoid phenolic glycosides (SPGs)—ecologically important compounds with significant commercial, cultural, and medicinal applications—remain largely undescribed. We used a linkage map derived from a full‐sib population of hybrid cottonwoods (Populus spp.) to search for quantitative trait loci (QTL) for the SPGs salicortin and HCH‐salicortin. SSR markers and primer sequences were used to anchor the map to the V3.0 P. trichocarpa genome. We discovered 21 QTL for the two traits, including a major QTL for HCH‐salicortin (R2 = .52) that colocated with a QTL for salicortin on chr12. Using the V3.0 Populus genome sequence, we identified 2,983 annotated genes and 1,480 genes of unknown function within our QTL intervals. We note ten candidate genes of interest, including a BAHD‐type acyltransferase that has been potentially linked to PopulusSPGs. Our results complement other recent studies in Populus with implications for gene discovery and the evolution of defensive chemistry in a model genus. To our knowledge, this is the first study to use a full‐sib mapping population to identify QTL intervals and gene lists associated with SPGs.

基因组学研究已被广泛用于鉴定调控多种重要植物次生代谢通路的基因。然而，柳苷类酚苷（salicinoid phenolic glycosides, SPGs）作为兼具重要商业、文化与药用价值的生态关键化合物，其相关编码基因迄今仍未得到充分解析。本研究利用源自杂交杨（Populus spp.）全同胞群体的连锁图谱，针对SPGs成分水杨苷（salicortin）与HCH-水杨苷（HCH-salicortin）搜寻数量性状基因座（quantitative trait loci, QTL）。研究使用简单序列重复（simple sequence repeat, SSR）标记与引物序列，将该连锁图谱锚定至毛果杨（P. trichocarpa）V3.0版本参考基因组。本研究共鉴定出与这两个性状相关的21个QTL，其中HCH-水杨苷的主效QTL（决定系数R²=0.52）与12号染色体上调控水杨苷的QTL存在共定位。借助毛果杨V3.0基因组序列，我们在上述QTL区间内共鉴定出2983个已注释基因与1480个功能未知基因。本研究筛选出10个候选功能基因，其中包括1个此前被推测与杨属SPGs合成相关的BAHD型酰基转移酶（BAHD-type acyltransferase）。本研究结果可为杨属相关研究提供补充，可为这一模式木本属的基因发掘与防御化学演化研究提供参考。据我们所知，本研究是首个利用全同胞作图群体鉴定与SPGs相关QTL区间及基因列表的研究。