CryoEM of bacterial conjugation proteins

Functional genetic analysis of the tcp locus has revealed that it mediates the conjugative transfer of the toxin and antibiotic resistance plasmids from Clostridium perfringens. This locus encodes thirteen genes that are required for efficient conjugative transfer of the paradigm tetracycline resistance plasmid, pCW3. The aim of this study is to determine the molecular architecture of the pCW3 DNA transfer machinery

针对tcp基因座（tcp locus）的功能遗传分析表明，该基因座可介导产气荚膜梭菌（Clostridium perfringens）所携带的毒素与抗生素抗性质粒的接合转移。该基因座编码13个基因，这些基因是实现模式四环素抗性质粒pCW3高效接合转移所必需的。本研究的目标是解析pCW3 DNA转移装置的分子架构。