Genes underlying leaf color transformation in ALS resistant sorghums

ALS-resistant sorghums (Sorghum bicolor (L.) Moench) were developed as a practical post-emergence weed control solution in sorghum. However, several ALS-resistant lines exhibit noticeable interveinal chlorosis in the early stages, leading to reduced vigor. Despite these lines eventually recovering and greening up at an advanced seedling stage, growers might hesitate to adopt ALS-resistant sorghums due to this atypical seedling phenotype. The goal was to identify plant mechanisms related to this unusual phenotype and to establish a foundation for addressing this issue. Two ALS-resistant genotypes, one displaying a yellow phenotype and the other a normal phenotype were cultivated, and tissue samples were collected at four time in-tervals, with the final sampling occurring after the genotypes had re-greened. RNA was extracted from these tissue samples and subjected to RNA-seq analysis.

抗ALS除草剂高粱（ALS-resistant sorghums，学名Sorghum bicolor (L.) Moench）被研发为高粱田实用的苗后杂草防控手段。然而，部分ALS抗除草剂品系在生长早期会出现显著的脉间失绿症状，进而导致植株长势减弱。尽管此类品系最终可在幼苗生长后期恢复并转绿，但由于这种非典型的幼苗表型，种植者往往对推广该类抗除草剂高粱持犹豫态度。本研究旨在鉴定与该异常表型相关的植株调控机制，并为解决该问题奠定研究基础。本研究培育了两株ALS抗除草剂基因型材料，一株表现为黄色表型，另一株为正常表型；并在四个时间节点采集组织样本，最后一次采样在两种基因型完成复绿后进行。从上述组织样本中提取总RNA，并开展RNA测序（RNA-seq）分析。