CUT&RUN Targeting SALL4 and HDAC2 in human melanoma cells. CUT&RUN Targeting SALL4 and HDAC2 in human melanoma cells

SALL4 builds a complex with histone deacetylases, and is thought to confer its effects epigenetically. Both loss of Sall4 and inhibition of HDAC2 leads to an invasiveness in human melanoma cells. To study co-occupancy of SALL4 and HDAC2, we employed CUT&RUN targeting SALL4 and HDAC2 in human melanoma cells (M010817).

SALL4可与组蛋白去乙酰化酶形成复合物，被认为通过表观遗传机制发挥生物学效应。敲除SALL4或抑制组蛋白去乙酰化酶2（HDAC2），均可诱导人黑色素瘤细胞产生侵袭性。为探究SALL4与HDAC2的共占据情况，我们在人类黑色素瘤细胞系M010817中采用了靶向SALL4和HDAC2的CUT&RUN技术。