H3K36me3 profiling in mTEClo

AIRE-sensitive genes are marked with low levels of H3K36me3 in mTEClo Overall design: Nano-ChIP-seq on H3K36me3 was performed as previously described (Adli and Bernstein, 2011) on 5*10^4 purified mTEClo (EpCAM+ UEA-1+ Ly51lo CD80lo) from individual B6 mice. ChIP-seq libraries were prepared with the TruSeq ChIP Sample Preparation Kit (Illumina) and 2x75-bp paired-end reads were sequenced on an Illumina HiSeq 2000 machine.

自身免疫调节因子（AIRE）敏感基因在Ly51低表达的髓质胸腺上皮细胞（mTEClo）中呈现低水平的H3K36me3修饰。整体实验设计：针对H3K36me3的纳米染色质免疫共沉淀测序（Nano-ChIP-seq）实验参照已发表方法（Adli与Bernstein，2011）完成，实验材料为单只B6小鼠来源的5×10^4个纯化的mTEClo细胞，其表型为EpCAM+ UEA-1+ Ly51lo CD80lo。ChIP-seq文库采用TruSeq ChIP Sample Preparation Kit（Illumina）构建，并通过Illumina HiSeq 2000测序仪完成2×75 bp双端读长测序。