Additional file 4 of Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology

Additional file 4. Table of individual mutants present in the REMI grid. Individual mutants were identified in 96-well plates using the REMI-seq technique (see methods). To identify the exact insertion points, the extracted tags were compared to the lookup table. The unique indices at the end of the inserts also allowed the determination of the vector version (p-GWDI G1 – G5; C6 – C8) as well as the orientation of the insert.

附加文件4：REMI网格中的单个突变体表格。本研究采用REMI-seq技术（详见方法部分）在96孔板中完成单个突变体的鉴定。为确定精确的插入位点，将提取得到的标签与查找表进行比对。插入片段末端的独特索引还可用于判定载体版本（p-GWDI G1–G5；C6–C8）以及插入片段的取向。