Kinetic parameters of SOD1 aggregation in the presence of different concentrations of Cu2+ as determined by 90o light scattering assays at 37°C.

The maximum slope of the best fit polynomial (dP/dt)max, kmax, was determined by fitting 90° light scattering intensity versus time to a seventh order polynomial equation. LS(∞) was determined by fitting 90° light scattering intensity versus time to the empirical Hill equation. k1, k2, LSmax,1, and LSmax,2 were determined by fitting 90° light scattering intensity versus time to a double exponential model. The final concentration of SOD1 was 10 µM. The buffer used was 20 mM Tris-HCl buffer (pH 7.4). Errors shown are standard errors of the mean.∼, observed from the 90° light scattering curves directly.ND, not determined because the 90° light scattering data in the present conditions could be fitted to such a double exponential model with a huge error.

通过将90°光散射强度（90° light scattering intensity）随时间的变化数据拟合至七阶多项式方程，得到最优拟合多项式的最大斜率[(dP/dt)max，即kmax]。通过将90°光散射强度随时间的变化数据拟合至经验希尔方程（empirical Hill equation），可求得LS(∞)。通过将90°光散射强度随时间的变化数据拟合至双指数模型（double exponential model），可求得k1、k2、LSmax,1及LSmax,2。实验中SOD1（SOD1）的终浓度为10 μM，所用缓冲液为20 mM Tris-HCl缓冲液（pH 7.4）。文中标注的误差为均值标准误（standard errors of the mean）。符号“~”表示直接从90°光散射曲线中观测得到的数值；符号“ND”表示未测定，这是由于当前实验条件下的90°光散射数据虽可拟合至该双指数模型，但拟合误差极大。