Chicory – a functional food with multiple effects: cancer-prevention, antimicrobial resistance, glycemia and lipidemia regulation. Chicory – a functional food with multiple effects: cancer-prevention, antimicrobial resistance, glycemia and lipidemia regulation

We experimented on murine model and attempted to point several mechanisms by which the chicory roots acts as a functional food. To this goal, a nutrigenomic analysis was performed. Overall design: Mice were fed with an aqueous decoction of the roots flour from two selected chicory genotypes (G12 and G35) and with an inulin (I2255 Sigma Aldrich, MO, USA) solution. The mice gavages consisted in a daily force-feeding of 500 µl of stemming solution decoctions of chicory flour or inulin water solution besides the standard chow. As experiments were conducted sequentially for two different periods of time, two control sets were used: first control group (Ctr1) in parallel with G12 and G35 diet and a second control group (Ctr2) in parallel with inulin diet. Three mice for each condition were sacrificed and the central core of the liver left lobe was cubed and frozen at -80 °C for transcriptomics.

本研究以小鼠模型为实验对象，旨在揭示菊苣根作为功能性食品的潜在作用机制。为此，本研究开展了营养基因组学（nutrigenomics）分析。
实验设计：实验小鼠分别饲喂两种筛选得到的菊苣基因型（G12、G35）根粉水煎剂，以及菊糖（inulin，货号I2255，西格玛奥德里奇（Sigma Aldrich），美国密苏里州）水溶液。小鼠灌胃方案为：在饲喂标准饲料的基础上，每日强制灌胃500 μl菊苣根粉水煎剂或菊糖水溶液。因实验分两个不同时段依次开展，故设置两组对照：第一对照组（Ctr1）与G12、G35日粮组平行对照，第二对照组（Ctr2）与菊糖日粮组平行对照。每个实验条件下取3只小鼠实施安乐死，采集其肝左叶中央区域组织，切成小块后置于-80℃冰箱冻存，用于转录组学（transcriptomics）分析。