Multifunctional material enables ultrafast and large-scale enrichment of glycopeptides and reveals distinct spatial patterns in oxidative stress

Our study offers a convenient and robust site-specific chemproteomics tool for glycoproteome profiling, enabling simultaneous identification of O-GlcNAc sites and N-glycosites by a two-step enzymatic release strategy, and the application of this material revealed distinct spatial O-GlcNAcylation patterns upon oxidative stress.

本研究开发了一款便捷且稳健的位点特异性化学蛋白质组学工具，用于糖蛋白质组分析。该工具借助两步酶解释放策略，可同时实现O-连接β-N-乙酰葡糖胺修饰位点（O-GlcNAc sites）与N-糖基化位点的鉴定。将该工具应用于相关研究后，研究团队揭示了氧化应激条件下独特的空间O-连接β-N-乙酰葡糖胺修饰分布模式。