Targeting Immune-Fibroblast Crosstalk in Myocardial Infarction and Cardiac Fibrosis II. Targeting Immune-Fibroblast Crosstalk in Myocardial Infarction and Cardiac Fibrosis II

To examine whether commonly used human cultured fibroblast systems model human cardiac fibroblasts, we tested three different fibroblast cell preparations: primary human cardiac fibroblasts (NHCF), human dermal fibroblasts (NHDF), and immortalized human cardiac fibroblasts (iHCF). Overall design: Each fibroblast cell line was treated with vehicle, TGF-β, or IL-1β for 72 hours and scRNAseq performed. Multiple biological and technical replicates were included, individually hash tagged, and pooled to minimize any potential batch effects.

为验证常用的人类培养成纤维细胞系统能否作为人心成纤维细胞的研究模型，我们选取了三种不同的成纤维细胞制剂开展实验：原代人心成纤维细胞（NHCF）、人皮肤成纤维细胞（NHDF）以及永生化人心成纤维细胞（iHCF）。总体实验设计如下：将每种成纤维细胞系分别以溶剂对照、转化生长因子-β（TGF-β）、白细胞介素-1β（IL-1β）处理72小时，随后进行单细胞RNA测序（scRNAseq）。本研究设置了多组生物学重复与技术重复，所有样本均单独完成哈希标签标记后混合，以最大限度减少潜在的批次效应。