Sertraline has fungicidal activity against Candida spp. and acts by inhibiting membrane and cell wall biosynthesis - Supplementary table 1

Aim: Our study evaluated the activity of sertraline (SER) alone and associated with antifungal drugs in planktonic Candida spp. strains, and investigated its mechanism of action. Materials & methods: Broth microdilution method and minimum fungicidal concentration/MIC ratio were used to assess SER anticandidal activity, and the interaction with antifungals was determined by fractional inhibitory concentration index. The mechanism of action was investigated by flow cytometry and in silico tests. Results: SER inhibited Candida spp. strains at low concentrations by the fungicidal effect and showed no loss of effectiveness when combined. Its action seemed to be related to the membrane and cell wall biosynthesis inhibition. Conclusion: SER has activity against Candida spp. isolated and associated with antifungals, and acts by causing cell wall and membrane damage.

研究目的：本研究评估了舍曲林（sertraline，SER）单独使用及与抗真菌药物联合使用对浮游态念珠菌属菌株的活性，并探究其作用机制。材料与方法：采用肉汤微量稀释法以及最小杀菌浓度（minimum fungicidal concentration）/最低抑菌浓度（MIC）比值评估舍曲林的抗念珠菌活性，并通过分级抑菌浓度指数测定其与抗真菌药物的相互作用。作用机制通过流式细胞术及计算机模拟实验进行探究。结果：舍曲林可在低浓度下通过杀菌作用抑制念珠菌属菌株，且联合使用时未出现药效降低。其作用机制似乎与抑制细胞膜及细胞壁生物合成相关。结论：舍曲林对分离得到的念珠菌属菌株具有抗菌活性，且可与抗真菌药物联合应用，其作用途径为造成细胞壁与细胞膜损伤。