Bacterial 16S rRNA gene sequencing in Kuroshio region east of Taiwan

A total of 78 samples were collected in June of 2013, March, July, and November of 2014, and March of 2015 by GoFlo bottles equipped with CTD (Sea-Bird Electronics, Bellevue, WA, USA) along a transect of 8 stations in the Kuroshio region between 121.72° and 123° E at 23.75° N east of Taiwan. To compare bacteria communities in vertical dimension, we collected water samples from both surface and deep chlorophyll a maximum (DCM) layers. During each sampling, ~20L water at each layer was first passed through a 1.2 um-pore-size polycarbonate filter (Durapore, Millipore, Billerica, MA, USA) to exclude eukaryotes and other larger particles, and then through a 0.2 um-pore-size filter to retain the bacterioplankton community. The sample processing was completed within 4 hours after water collection. After filtration, the 0.2 um-pore size filters were stored in liquid nitrogen onboard and then shift to -20°C until further molecular analysis.本数据集共包含78份水样样品，采集时间为2013年6月、2014年3月、7月、11月及2015年3月，采样载体为搭载CTD（电导-温度-深度仪，Sea-Bird Electronics公司，美国华盛顿州贝尔维尤市）的GoFlo采水器，采样断面位于台湾以东23.75°N、121.72°E至123°E的黑潮海域，共设置8个站位。为对比垂向维度下的细菌群落结构，我们分别采集了表层与深层叶绿素a最大值（DCM）层的水样。每次采样时，先将各层约20升水样通过孔径1.2微米的聚碳酸酯滤膜（Durapore，密理博公司，美国马萨诸塞州比勒里卡市）以去除真核生物及其他大颗粒杂质，随后再通过孔径0.2微米的滤膜截留浮游细菌群落。所有样品处理均在水样采集后4小时内完成。过滤结束后，将孔径0.2微米的滤膜置于液氮中现场保存，随后转移至-20℃环境保存，直至开展后续分子生物学分析。