Gene Expression During Recovery from Freezing

Freezing causes physiological changes even in a hardy, cold-acclimated wild type. During recovery from freezing, gene expression will reflect the induction of damage-repair processes distinct from the damage-prevention associated with cold acclimation. This will be detected by observing the wild-type transcriptome at two time points during recovery from a freezing episode. The appropriate control is the unfrozen, cold-acclimated wild type. Experimenter name = Glenn Thorlby Experimenter phone = 01874 443770 Experimenter address = School of biological Sciences Experimenter address = Bourne Building Experimenter address = Royal Holloway Experimenter address = Egham Experimenter zip/postal_code = TW20 0EX Keywords: growth_condition_design; 3 samples were used in this experiment即使是耐受性优良且经冷驯化的野生型（wild type）个体，冷冻应激也会引发其生理变化。在从冷冻应激中恢复的过程中，基因表达将体现出损伤修复程序的诱导激活——这一过程与冷驯化相关的损伤预防机制截然不同。该差异可通过观测野生型转录组（transcriptome）在一次冷冻应激后的恢复阶段的两个时间点的表达情况得以检测。本实验的适宜对照组为未经历冷冻的冷驯化野生型个体。 实验者姓名：格伦·索思比（Glenn Thorlby） 实验者联系电话：[phone] 实验者通讯地址：生命科学学院、伯恩大楼、皇家霍洛威、伊格姆 邮政编码：TW20 0EX 关键词：生长条件设计（growth_condition_design）；本实验共使用3份样本。