Replication Data for: Impact of rAAV-shRNA treatment targeting mechanosensitive Ilk1 and Fermt2 in a mouse model of sepsis-induced muscle weakness

We hypothesized that the integrin-receptor-complex through key elements KIND2 and ILK, plays a role in sepsis-induced-muscle-weakness. AAV2/9-vectors expressing shRNA-sequences against Ilk1, Fermt2 or noncoding-control-gene were injected in tibialis anterior (TA) muscles of 24w-old male C57BL/6J mice. Two-weeks-post-injection, after knockdown validation, mice were made septic by cecal ligation and puncture. Five-days-post-sepsis muscle force, mass and fiber size were quantified and expression of mechanosensitive elements and downstream pathways of the integrin-receptor-complex was assessed.

本研究假设，整合素受体复合物（integrin-receptor-complex）通过关键元件KIND2与ILK，在脓毒症诱导的肌肉无力中发挥调控作用。研究者向24周龄雄性C57BL/6J小鼠的胫骨前肌（tibialis anterior, TA）中注射搭载靶向Ilk1、Fermt2或非编码对照基因的短发夹RNA（short hairpin RNA, shRNA）序列的AAV2/9载体。注射两周后，经敲低效率验证，研究者通过盲肠结扎穿刺术构建脓毒症小鼠模型。脓毒症造模五天后，对小鼠的肌肉力量、肌肉质量及肌纤维尺寸进行定量检测，并评估整合素受体复合物的机械敏感元件及其下游通路的表达水平。