Raw data of SDS-PAGE gels and microscopy for the research paper "Mapping Polyamine-Protein Interactions in Live Cells Through Photoaffinity Labeling"

Polyamines are essential metabolites that play a crucial role in regulating key cellular processes. While previous studies have shown that polyamines modulate protein function through non-covalent interactions, the lack of robust analytical methods has limited the systematic identification of these interactions in living cells. To address this challenge, we synthesized novel photoaffinity probes and applied them to a model cell line, identifying over 400 putative protein interactors with remarkable structure-dependent specificity. The interaction profiles of these probes were visualized through in-gel fluorescence scanning, and their subcellular localization was examined using fluorescence microscopy. Higher polyamine analogs predominantly target proteins in the nucleoplasm and cytosol, whereas diamine analogs localize to vesicle-like structures near the Golgi apparatus, implying that different polyamine types show a proclivity for specific cellular compartments. This study provides a comprehensive overview of polyamine-protein interactions in live cells, offering valuable insights into their roles in cellular processes. This dataset contains unedited and uncropped gel and microscopy images used in the publication and electronic supplementary information. This research was funded by the National Science Centre, Poland (2020/38/E/ST4/00250).

多胺（Polyamines）是一类至关重要的代谢物，在调控核心细胞过程中发挥关键作用。尽管既往研究已证实多胺可通过非共价相互作用调控蛋白质功能，但缺乏可靠的分析方法限制了活细胞内此类相互作用的系统性鉴定。为应对这一挑战，本研究合成了新型光亲和探针（photoaffinity probe），并将其应用于模型细胞系，成功鉴定出400余种具有显著结构依赖性特异性的推定蛋白质相互作用靶点。通过凝胶内荧光扫描可视化这些探针的相互作用图谱，并借助荧光显微镜检测其亚细胞定位。高级多胺类似物主要靶向核质与细胞质中的蛋白质，而二胺类似物则定位于高尔基体（Golgi apparatus）附近的囊泡样结构，这表明不同类型的多胺倾向于靶向特定的细胞区室。本研究全面阐明了活细胞内多胺与蛋白质的相互作用，为解析其在细胞进程中的功能提供了宝贵见解。本数据集包含发表论文及电子补充材料中使用的未编辑、未裁剪的凝胶与显微镜图像。本研究获得波兰国家科学中心（National Science Centre, Poland）资助（项目编号：2020/38/E/ST4/00250）。