Single-cell RNA sequencing of murine kidneys for ischemia-reperfusion induced renal injury and fibrosis. Single-cell RNA sequencing of murine kidneys for ischemia-reperfusion induced renal injury and fibrosis

Using single-cell RNA sequencing (scRNA-seq), we isolated approximately 100,000 cells from mouse kidneys and generated a large-scale transcriptomic dataset corresponding to time points of 4 hours, 12 hours, 1 day, 5 days, 14 days, and 28 days after IR. Our preliminary analysis identified 16 distinct cell types, providing a robust database to study the changes and evolution of renal IRI from early to late stages. Overall design: C57BL/6 mice were euthanized at 4 hours, 12 hours, 1 day, 5 days, 14 days, and 28 days after IR, and analyzed using scRNA-seq.

采用单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）技术，我们从小鼠肾脏中分离得到约100,000个细胞，并构建了对应缺血再灌注（ischemia-reperfusion, IR）后4小时、12小时、1天、5天、14天及28天多个时间点的大规模转录组数据集。本研究通过初步分析鉴定出16种不同的细胞类型，为研究肾缺血再灌注损伤（renal ischemia-reperfusion injury, renal IRI）从早期至晚期的动态变化与演化进程提供了可靠的数据库。整体实验设计：将C57BL/6小鼠在缺血再灌注（IR）后4小时、12小时、1天、5天、14天及28天实施安乐死，并采用scRNA-seq技术进行分析。