Dysfunction of 5-methylcytosine oxidases and the Polycomb protein RYBP leads to widespread DNA hypermethylation and cell transformation [ChIP-seq]

We assessed the levels of the PRC1 Polycomb mark, RYBP, H2AK119Ub1, and the PRC2 Polycomb mark, H3K27me3, considering the extensive crosstalk between PRC1 and PRC2 activities. Chromatin immunoprecipitation sequencing (ChIP-seq) for RYBP, H2AK119ub1 and H3K27me3 in HBEC3 defective cell (Control cells, RYBP KO, YAF2 KO, RYBP/YAF2 DKO).

鉴于多梳抑制复合体1 (Polycomb Repressive Complex 1，PRC1)与多梳抑制复合体2 (Polycomb Repressive Complex 2，PRC2)的活性存在广泛的串扰调控，我们通过染色质免疫共沉淀测序 (Chromatin immunoprecipitation sequencing，ChIP-seq) 技术，对HBEC3缺陷型细胞（含对照细胞、RYBP基因敲除细胞、YAF2基因敲除细胞及RYBP/YAF2双基因敲除细胞）中PRC1相关多梳修饰标记RYBP、组蛋白H2A赖氨酸119单泛素化 (H2AK119Ub1)，以及PRC2相关多梳修饰标记组蛋白H3赖氨酸27三甲基化 (H3K27me3) 的水平进行了检测。