High-throughput sequencing in ARID1A knockout MDA-MB-231 cells [H3K4me3 histone modification ChIP-seq]. High-throughput sequencing in ARID1A knockout MDA-MB-231 cells [H3K4me3 histone modification ChIP-seq]

ARID1A has been suggested as a key regulator for anti-tumor immunity. In the present study, we evaluated the role of ARID1A deficiency in inducing adaptive immune resistance in triple negative breast cancer. To explore global transcriptional activity, we performed chromatin immunoprecipitation followed by high-throughput sequencing (ChIP–seq) to investigate H3K4me3 histone modification of ARID1A knockout and control MDA-MB-231 cells. Overall design: We performed H3K4me3 ChIP-seq in ARID1A knockout and control MDA-MB-231 cells.

ARID1A基因（ARID1A）已被认为是抗肿瘤免疫的关键调控因子。本研究中，我们评估了ARID1A缺失在三阴性乳腺癌中诱导适应性免疫抵抗的作用。为探究全局转录活性，我们开展了染色质免疫沉淀联合高通量测序（ChIP–seq）实验，以检测ARID1A敲除及对照MDA-MB-231细胞的H3K4me3组蛋白修饰情况。实验设计概述：我们在ARID1A敲除及对照MDA-MB-231细胞中开展了H3K4me3 ChIP-seq实验。