Additional aspartate leads to a production of bigger cells and more C-period cells.

Exponentially growing cells in ABT medium supplemented with amino acids as indicated were fixed in 70% ethanoland then analyzed by flow cytometry as described in Materials and Methods. The number of cells in B-, C- and D-period was calculated using a software provided by BD and cell size was determined by fluorescent microscopy. Each calculation includes more than 100 cells. The values are average of three individual experiments, standard errors are given.

将在按指定条件补充氨基酸的ABT培养基中培养的指数生长期细胞用70%乙醇固定，随后按照材料与方法中所述流程进行流式细胞术分析。采用BD公司提供的软件统计B期、C期及D期的细胞数目，通过荧光显微镜测定细胞尺寸。每一次统计均包含超过100个细胞，所有数值为三次独立重复实验的平均值，并标注了标准误。