Reduced Representational Bisulfite Sequencing of R1 and Dnmt1 Tet/Tet mouse embryonic stem cell lines. Reduced Representational Bisulfite Sequencing of R1 and Dnmt1 Tet/Tet mouse embryonic stem cell lines

We generated RRBS data to enable a comparative DNA methylation analysis of Dnmt1 Tet/Tet, a mouse embyonic stem cell line (ESC) that overexpresses DNMT1 and the WT R1 ESCs and their neuronal derivatives. The optimized data analysis workflows provides a framework for comparative investigations of methylation profiles. This platform offers a comprehensive and more accurate quantitative and qualitative evaluation of methylated cytosines in ESCs and neurons. We expect that RRBS-based methylome analysis permits to understand the epigenetic basis of abnormal neurogenesis. Overall design: Study of the methylation profiles of R1 and Dnmt1 Tet/Tet ESCs and their neuronal derivatives.

我们生成了简化代表性亚硫酸氢盐测序（Reduced Representation Bisulfite Sequencing, RRBS）数据，以开展针对过表达DNA甲基转移酶1（DNA methyltransferase 1, DNMT1）的Dnmt1 Tet/Tet小鼠胚胎干细胞（embryonic stem cell, ESC）系、野生型（wild type, WT）R1胚胎干细胞及其神经分化细胞的DNA甲基化比较分析。本研究优化的数据分析工作流程为甲基化图谱的比较研究提供了分析框架。该平台可对胚胎干细胞及神经元中的甲基化胞嘧啶实现全面且更为精准的定量与定性评估。我们预期基于RRBS的甲基化组分析将有助于解析异常神经发生的表观遗传基础。整体实验设计：针对R1与Dnmt1 Tet/Tet胚胎干细胞及其神经分化细胞的甲基化图谱展开研究。