Bromodomain protein inhibition protects β-cells from cytokine-induced death and dysfunction via antogonism of NF-κB pathway. Bromodomain protein inhibition protects β-cells from cytokine-induced death and dysfunction via antogonism of NF-κB pathway

We report that BET inhibitor (I-BET762) protect against cytokine induced pancreatic beta-cell apoptosis and did RNA-Seq to determine global transcriptome changes with the drug. Overall design: Rat insulinoma cell line (INS1) were treated with vehicle control (VC), I-BET762 (IB), ctyokine with vehicle control (CCVC) and cytokines with I-BET762 (CCIB) for 72h with cytokines added in last 24h. contributor: Novogene

本研究报道，BET抑制剂（BET inhibitor）I-BET762可拮抗细胞因子（cytokine）诱导的胰腺β细胞凋亡；本研究通过RNA-Seq技术，解析该药物处理后细胞的全局转录组变化。
整体实验设计：以大鼠胰岛素瘤细胞系（INS1）为研究对象，设置四组处理：溶剂对照（vehicle control，VC）组、I-BET762（IB）组、细胞因子+溶剂对照（CCVC）组以及细胞因子+I-BET762（CCIB）组；所有处理均持续72小时，且细胞因子于最后24小时添加。
项目贡献单位：诺禾致源（Novogene）