Use of low density lipoprotein in different concentrations in diluent medium for cryopreservation of ovine semen

ABSTRACT The use of egg yolk makes it difficult to standardize extenders and presents biological hazards. Thus, this study evaluated different concentrations of low-density lipoprotein (LDL) to replace yolk extenders for production of sperm for cryopreservation in ovine. A control extender was used (CTR= 20% yolk) and five treatments, replacing the yolk by different ratios of LDL (T1= 6%; T2= 8%, T3= 12%; T4= 16%; T5= 20%) all based on TRIS-glycerol. For the study, two ejaculates from six Santa Ines breeding were used. Sixty days after cryopreservation, the samples were thawed and evaluated for total motility (MT, %) and progressive motility (MP, %), vigor (1-5) and the functional integrity (hyposmotic shock with distilled water, %) and structural (supravital dye eosin, %) of the sperm membranes. The evaluations of strength and membrane functionality didn’t differ (P> 0.05) between groups. However, T4 (P

摘要 蛋黄的使用会导致稀释液难以标准化，且存在生物危害风险。因此，本研究评估了不同浓度的低密度脂蛋白（low-density lipoprotein, LDL）替代蛋黄稀释液用于绵羊精子冷冻保存的生产效果。实验设置了对照稀释液组（CTR组：含20%蛋黄），以及5个以不同比例低密度脂蛋白替代蛋黄的处理组（T1=6%；T2=8%、T3=12%；T4=16%；T5=20%），所有稀释液均基于TRIS-甘油体系。本研究采集了6只圣塔伊内斯（Santa Ines）种公羊的2份精液样本。冷冻保存60天后，对样本进行解冻并检测以下指标：总活力（MT，%）、前向运动活力（MP，%）、活力等级（1-5分），以及精子膜的功能完整性（蒸馏水低渗肿胀试验，%）与结构完整性（伊红超活染色法，%）。各组间的精子活力与膜功能完整性无显著差异（P>0.05）。然而，T4组（P