Optimum of PBMC cell proliferation was at 400 setae/micro-well.

PBMC from one donor previously exposed to setae was incubated with different numbers of setae medium (0 setae), 200 setae, 400 setae and 800 setae/micro- well. Setae were suspended in PBS and sonicated before stimulation and cell proliferation was determined by 3H-thymidine incorporation. The experimental setup is described in material and methods. The mean ct/min of 4–5 parallel incubations ± standard deviation (SD) are given.
Optimum of PBMC cell proliferation was at 400 setae/micro-well.

将此前接触过刚毛（setae）的单名供体的外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMC），与含不同数量刚毛的培养基（0个刚毛、200个刚毛、400个刚毛及800个刚毛/微孔）共同孵育。实验前，刚毛悬浮于磷酸盐缓冲液（Phosphate Buffered Saline, PBS）中并经超声处理，随后用于细胞刺激；细胞增殖水平通过3H-胸腺嘧啶核苷掺入法进行检测。实验具体设置详见材料与方法部分。本研究给出4~5份平行孵育样本的平均每分钟计数（ct/min）±标准差（Standard Deviation, SD）。
PBMC细胞增殖的最适条件为每微孔400个刚毛。