Expression data from Myotonic dystrophy type 1 (DM1) patient-derived induced pluripotent cell

DM1-iPSCs were generated from the peripheral blood of male adult patients. All clones were generated using episomal vectors. For skeletal muscle differentiation of the DM1-iPSCs, tet-on MYOD1 expression vector was transfected into DM1-iPSCs. To examine whether DM1-iPSC clones with different repeat sizes have acquired different characteristics, we isolated DM1-iPSC clones with different repeat sizes. We isolated 2 clones (S45 and S118) showing repeat size of 400~900, 2 clones (S14 and S24) showing repeat size of 1200~1600, and 2 clones (S1 and S16) showing repeat size of 1800~2800. Cells were harvested at undifferentiated stages for RNA extraction and hybridization on Affymetrix microarrays. We performed gene expression profiles of 6 DM1-iPSC subclones and 1 healthy control iPS cell (normal).

本数据集所用的DM1型诱导多能干细胞（DM1-induced pluripotent stem cells, DM1-iPSCs）源自成年男性患者的外周血，所有细胞克隆均通过附加型载体（episomal vector）构建获得。为诱导DM1-iPSCs向骨骼肌细胞分化，我们将四环素诱导型（tet-on）MYOD1表达载体转染至DM1-iPSCs中。为探究不同重复序列拷贝数的DM1-iPSC克隆是否具备差异化生物学特性，我们筛选得到了携带不同重复序列拷贝数的DM1-iPSC克隆：其中重复序列拷贝数范围为400~900的克隆共2株（S45、S118），1200~1600的克隆共2株（S14、S24），1800~2800的克隆共2株（S1、S16）。我们于细胞未分化阶段收集样本，用于RNA提取及Affymetrix基因芯片杂交实验，并完成了6株DM1-iPSC亚克隆与1株健康对照诱导多能干细胞（正常iPSCs）的基因表达谱分析。