Expression data of osteogenic differentiated cartilage endplate-derived stem cells (CESCs) under hypoxia or normoxia

Cartilage endplate-derived stem cells (CESCs) with chondro-osteogenic differentiation capacity may be responsible for the balance of chondrification and ossification in cartilage endplate (CEP). CEP is an avascular and hypoxic tissue, and hypoxia could inhibit the osteogenic differentiation of CESCs. We used high-throughput scanning to identify differentially expressed genes (DEGs) and alternatively spliced genes (ASGs) during osteogenic differentiation of CESCs under hypoxia compared to those induced under normoxia. Human cartilage endplate-derived stem cells (CESCs) were treated with osteogenic differentiation medium under normoxia and hypoxia for 21 days respectively.

软骨终板源性干细胞（cartilage endplate-derived stem cells, CESCs）具备成软骨-成骨分化潜能，或可参与维持软骨终板（cartilage endplate, CEP）内软骨化与骨化的动态平衡。软骨终板属于无血管低氧组织，低氧可抑制CESCs的成骨分化。本研究采用高通量扫描技术，鉴定出低氧条件下CESCs成骨分化过程中，相较于常氧诱导组的差异表达基因（differentially expressed genes, DEGs）与可变剪接基因（alternatively spliced genes, ASGs）。实验中将人软骨终板源性干细胞分别置于常氧与低氧环境中，使用成骨分化培养基培养21天。