Data underlying the publication: “Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation”

The research objective was to quantify at the single-molecule level the motion of fully in vitro reconstituted CMG, the eukaryotic replicative helicase. <br> This was achieved by using a combination of optical tweezers and confocal fluorescence microscopy. The position along DNA molecules held in an optical trap of fluorescently labeled CMG was tracked over time, and different motion parameters were studied (e.g. diffusion constants and velocities) under different biochemical conditions.

本研究旨在于单分子水平上，定量分析完全体外重构的CMG——真核生物复制解旋酶的运动特性。
本研究通过结合光镊与共聚焦荧光显微镜技术实现了上述研究目标。
研究人员对光镊捕获的DNA分子上、经荧光标记的CMG的位置进行了实时追踪，并在不同生化条件下考察了多种运动参数（如扩散常数与运动速率）。