Nutrient requirements of M. oryzae Δmet6mutants.

Wild type (P1.2) and Δmet6 M. oryzae mutants (M15.1, M22.1, M23.1) were grown on minimal medium (MM) supplemented with different sulfur metabolites. Mycelium growth was observed 4 to 6 days after transfert of a mycelial plug from CM to MM. MTA was dissolved in dimethylsulfoxide (DMSO) and added to MM at 1% DMSO final concentration.+++, normal growth++, reduced growth+, highly reduced growth-, no growth.Nutrient requirements of M. oryzae Δmet6mutants.野生型菌株P1.2与Δmet6型稻瘟病菌（M. oryzae）突变体M15.1、M22.1、M23.1，均接种于添加不同硫代谢产物的基础培养基（minimal medium，MM）中培养。将菌丝块从完全培养基（complete medium，CM）转接至MM培养基后，于4至6天观察菌丝生长状况。甲硫腺苷（MTA）溶于二甲基亚砜（dimethylsulfoxide，DMSO）后，以终浓度为1% DMSO的比例添加至MM培养基中。生长评级标准如下：+++代表正常生长，++代表生长减弱，+代表生长大幅减弱，-代表无生长。本实验旨在探究稻瘟病菌Δmet6突变体的营养需求。