Inflammatory cytokine and prostacyclin release from HMVEC after stimulation of NOD1 or TLR4.

HMVEC were cultured for 24 hours in 96 well plates with media alone (CTRL), iE-DAP 10 µg/ml (NOD1) or LPS 1 µg/ml (TLR4). (A) Inflammatory cytokine release measured using the Human Pro-Inflammatory 9-PlexBase Kit from Meso Scale Discovery (MSD®). Results are expressed as mean ± SEM for n = 15. (B) 6-keto prostaglandin F1α release (breakdown product of Prostacyclin) measured by ELISA. Results are expressed as mean ± SEM for n = 15. Results analysed by one-way ANOVA with Dunnett’s multiple comparison test (*P#P

将人微血管内皮细胞（HMVEC）接种于96孔板中，分别以仅含基础培养基的空白对照（CTRL）、10 µg/ml的iE-DAP（激活NOD1通路）或1 µg/ml的脂多糖（LPS，激活TLR4通路）培养24小时。(A) 采用美索尺度发现（MSD®）出品的人类促炎因子9联基础试剂盒（Human Pro-Inflammatory 9-PlexBase Kit）检测炎症细胞因子释放水平，结果以平均值±标准误（SEM）表示，每组样本量n=15。(B) 采用酶联免疫吸附测定（ELISA）检测6-酮前列腺素F1α（前列环素的降解产物）的释放水平，结果以平均值±标准误（SEM）表示，每组样本量n=15。所有结果采用单因素方差分析（one-way ANOVA）结合Dunnett多重比较检验（Dunnett’s multiple comparison test）进行统计分析（*P#P）