Genotyping-by-sequencing of commercial and native Florida passion fruit species

The passion fruit genus, Passiflora, is diverse comprising more than 500 species with several species admired for their tropical fruit and ornate flowers. Commercially, P. edulis is the most important species and includes yellow or purple-skinned fruit types. Major threats to commercial production include both abiotic and biotic stresses that can reduce yield, decrease fruit quality, or kill passion fruit vines. Recent advances in passion fruit genomics are facilitating systematic plant improvement and the creation of elite cultivars by leveraging the high phenotypic and genetic diversity of the species. Marker-assisted breeding of passionfruit has the potential to enable informed decisions related to parental selection, confirm parentage of potential hybrids, identify mis-identified accessions, and resolve species assignments. The purpose of this study was to develop single nucleotide polymorphism (SNP) markers to characterize a passion fruit germplasm collection with an emphasis on native Florida passion fruit species. In total, 59 accessions from 20 species were genotyped.

西番莲属（Passiflora）物种多样性丰富，包含超过500个物种，其中多个物种因其热带果实与华丽花朵广受赞誉。商业种植中，鸡蛋果（P. edulis）是最为重要的物种，其果实分为黄皮与紫皮两个品类。西番莲商业生产面临的主要威胁包括非生物胁迫与生物胁迫，此类胁迫可降低产量、削弱果实品质，甚至导致西番莲藤蔓死亡。近年来西番莲基因组学领域的研究进展，正依托该物种丰富的表型与遗传多样性，推动系统性的作物改良与优良栽培品种的培育。西番莲标记辅助育种有望为亲本选择提供科学决策依据、验证潜在杂交组合的亲子关系、鉴定错误鉴定的种质材料，并厘清物种分类归属。本研究旨在开发单核苷酸多态性（SNP）标记，以表征西番莲种质资源收集库，研究重点聚焦佛罗里达州本土西番莲物种。本研究共对20个物种的59份种质资源进行了基因分型。