Replication Data for: Muscle proteome analysis of facioscapulohumeral dystrophy patients reveals a metabolic rewiring promoting oxidative/reductive stress contributing to the loss of muscle function

This dataverse include the 2-D DIGE and LC-MS/MS proteomics datasets of changed proteins generated in the study: “muscle proteome analysis of facioscapulohumeral dystrophy patients reveals a metabolic rewiring promoting oxidative/reductive stress contributing to the loss of muscle function” by Manuela Moriggi, Lucia Ruggiero, Enrica Torretta, Dario Zoppi, Beatrice Arosio, Evelyn Ferri, Alessandra Castegna, Chiara Fiorillo, Cecilia Gelfi and Daniele Capitanio. Data were generated by LC-ESI-MS/MS with label-free quantitation. Mass spectra were analyzed using MaxQuant software (Max-Planck-Institute of Biochemistry, Munich, Germany, version 1.6.17.0). Statistical analyses were performed using the Perseus software (Max Planck Institute of Biochemistry, Munich, Germany, version 1.6.15.0). For statistical analysis, Student's t-test with a p-value threshold of 0.05 was applied. To exclude the presence of false positives from the analysis, Benjamini–Hochberg false discovery rate test was applied.

本数据集涵盖下述研究中获取的差异表达蛋白质的二维差异凝胶电泳（2-D DIGE）与液相色谱-串联质谱（LC-MS/MS）蛋白质组学数据集：《面肩肱型肌营养不良症（facioscapulohumeral dystrophy）患者的肌肉蛋白质组学分析揭示代谢重编程促进氧化/还原应激，进而导致肌肉功能丧失》，作者为Manuela Moriggi、Lucia Ruggiero、Enrica Torretta、Dario Zoppi、Beatrice Arosio、Evelyn Ferri、Alessandra Castegna、Chiara Fiorillo、Cecilia Gelfi及Daniele Capitanio。数据集采用无标记定量液相色谱-电喷雾电离串联质谱（LC-ESI-MS/MS）技术生成。质谱数据由德国慕尼黑马克斯·普朗克生物化学研究所开发的MaxQuant软件（版本1.6.17.0）进行分析。统计分析采用德国慕尼黑马克斯·普朗克生物化学研究所开发的Perseus软件（版本1.6.15.0）完成。统计分析环节采用p值阈值为0.05的Student t检验；为排除分析中的假阳性结果，同时应用了Benjamini-Hochberg假发现率检验。