Transcriptomic signatures and chromatin structure in adipocytes isolated from premenauposal women subcutaneous fat [RNA-seq]. Transcriptomic signatures and chromatin structure in adipocytes isolated from premenauposal women subcutaneous fat [RNA-seq]

Our data showed that lipid and glucose metabolic pathways genes were expressed at higher levels in gluteofemoral adipocyte fraction in pears, while genes associated with inflammation were higher in both abdominal and gluteofemoral apple adipocyte fraction. Gluteofemoral adipocyte chromatin from pear-shaped women contained a significantly higher number of differentially open ATAC-seq peaks relative to chromatin from the apple-shaped gluteofemoral adipocytes. In contrast, abdominal adipocyte chromatin openness showed few differences between apple and pear-shaped women. We revealed a correlation between gene transcription and open chromatin at the proximity of the TSS of some of the differentially expressed genes. Overall design: Adipocytes isolated from Abdominal (A) and gluteofemoral (GF) adipose tissue obtained from premenopausal apple-shaped and pear-shaped women were used to perform transcriptome analysis by RNA-seq and assess accessible chromatin regions by ATAC-seq.

本研究数据显示，梨形身材人群的臀股部脂肪细胞组分中，脂质与葡萄糖代谢通路相关基因的表达水平更高；而苹果形身材人群的腹部及臀股部脂肪细胞组分中，炎症相关基因的表达水平均更高。与苹果形身材人群的臀股部脂肪细胞染色质相比，梨形身材人群的臀股部脂肪细胞染色质中，差异开放的转座酶可及性测序（ATAC-seq）峰数量显著更多。与之相反，苹果形与梨形身材人群的腹部脂肪细胞染色质可及性差异极小。本研究还发现，部分差异表达基因的转录起始位点（Transcription Start Site, TSS）附近区域的基因转录与开放染色质之间存在相关性。整体实验设计：从绝经前苹果形及梨形身材女性的腹部（A）与臀股部（GF）脂肪组织中分离得到脂肪细胞，通过RNA测序（RNA-seq）开展转录组分析，并通过ATAC-seq检测可及染色质区域。