Table_1_Mitigation of oxidative stress and inflammatory factors, along with the antibrowning and antimicrobial effects of cassia seed microbial fermentation solution.DOCX

IntroductionCassia seeds, originating from the mature seeds of leguminous cassia species, possess pharmacological effects attributed to their rich composition of various active ingredients, notably anthraquinones. While current research predominantly focuses on pharmaceutical extractions, there has been limited progress in fermentation studies.
MethodsOur study aimed to enhance the content of active compounds such as anthraquinones, flavonoids, and polyphenols using microbial fermentation techniques. We specifically optimized a fermentation system through a single-factor experimental design.
ResultsThe antioxidant properties of the fermentation solution were validated through assays involving HaCaT cells and zebrafish. We observed effective suppression of inflammatory reactions in both RAW264.7 cells and transgenic zebrafish by the fermentation solution. Moreover, significant inhibition of tyrosinase activity and melanin production was evident in B16-F10 cells and zebrafish. Positive outcomes were also obtained in antibacterial assays and chick embryo experiments.
DiscussionThese findings highlight the potential of cassia seed fermentation solution as a safe and eco-friendly material in food chemistry and biomedical sciences.

引言 决明子（Cassia seeds）为豆科决明属植物的成熟种子，因其富含多种活性成分（尤以蒽醌类（anthraquinones）物质为代表）而具备药理活性。当前相关研究多聚焦于药物提取领域，而发酵相关研究进展较为有限。
材料与方法 本研究旨在通过微生物发酵技术，提升决明子发酵液中蒽醌类（anthraquinones）、黄酮类（flavonoids）及多酚类（polyphenols）等活性成分的含量；并采用单因素实验设计对发酵体系进行优化。
结果 本研究通过HaCaT细胞与斑马鱼（zebrafish）实验验证了发酵液的抗氧化活性；观察到发酵液可有效抑制RAW264.7细胞与转基因斑马鱼的炎症反应；同时在B16-F10细胞及斑马鱼模型中，发酵液可显著抑制酪氨酸酶（tyrosinase）活性与黑色素生成。此外，抗菌实验与鸡胚实验均获得了阳性结果。
讨论 本研究结果显示，决明子发酵液作为一种安全环保的材料，在食品化学与生物医学领域具备良好的应用潜力。