RAMPAGE dataset for the human K562 cell line

5''-complete cDNA sequencing on ribosome-depleted total RNA from the human K562 cell line. Provides high-quality, genome-wide single-base resolution profiling of transcription start sites and their expression levels. Overall design: This dataset represents a whole-genome, single-base resolution profiling of transcription start site (TSS) expression in the human K562 cell line. These profiles were established using RAMPAGE, a high-throughput, high-accuracy 5''-complete cDNA sequencing method implemented on the Illumina platform. The data was analyzed using custom scripts and algorithms that are all available upon request.针对人类K562细胞系中经核糖体去除处理的总RNA开展5'端完整互补DNA（cDNA）测序。该方案可实现高质量、全基因组单碱基分辨率的转录起始位点（Transcription Start Site, TSS）及其表达水平谱分析。 整体实验设计：本数据集针对人类K562细胞系开展全基因组单碱基分辨率的转录起始位点（TSS）表达谱分析。该谱图通过RAMPAGE技术构建完成，这是一种在Illumina平台上实现的高通量、高精度5'端完整cDNA测序方法。本数据集的数据分析依托自定义脚本与算法完成，所有代码与算法均可应要求提供。