Evaluation of the assay system 3: ebony as the marker gene.

Evaluation of the assay system with ebony as the marker gene instead of lac2. The second instar larvae were categorized into the ebony phenotype (i.e. the second RNAi worked) and the WT phenotype (i.e. the second RNAi is suppressed). The suppression of the second RNAi phenotype by the initial RNAi was evaluated as the proportion of the WT larvae in the survived second instar larvae (Suppression).

本研究对以ebony为标记基因替代lac2的检测体系开展评估。将第二龄幼虫分为ebony（黑檀体基因）表型（即第二次RNA干扰（RNAi）成功）与野生型（Wild Type, WT）表型（即第二次RNA干扰受到抑制）。初始RNA干扰对第二次RNA干扰表型的抑制效果，以存活第二龄幼虫中野生型幼虫的占比作为量化评估指标，该指标记为抑制率（Suppression）。