Sequential Injection Analysis of Butyrylcholinesterase Using Butyrylcholine Ion-Selective Electrode Detector (Supporting Information)

A new automated analytical method for the determination of Butyrylcholinesterase (BChE) based on sequential injection analysis (SIA) method using a butyrylcholine chloride (BuCh) ion-selective electrode (ISE) detector was developed. BuCh is degraded to choline and butyrate by the enzymatic activity of BChE, resulting in a decrease in BuCh concentration. By detecting this change in BuCh concentration with BuCh-ISE, BChE can be determined indirectly. In the present SIA method using the BuCh-ISE detector based on α-cyclodextrin membrane, BChE in the concentration range of 0.015–0.50 units/mL was determined at a BuCh concentration of 2.0 mmol dm−3 and a reaction time of 5.0 min. The detection limit for BChE is 4.8 × 10−3 units/mL.本研究开发了一种基于顺序注射分析（sequential injection analysis, SIA）的新型自动化分析方法，用于丁酰胆碱酯酶（Butyrylcholinesterase, BChE）的定量检测，该方法以氯化丁酰胆碱（butyrylcholine chloride, BuCh）离子选择性电极（ion-selective electrode, ISE）作为检测器。丁酰胆碱酯酶可通过其酶促活性将氯化丁酰胆碱降解为胆碱与丁酸，导致氯化丁酰胆碱浓度降低。借助氯化丁酰胆碱离子选择性电极检测该浓度变化，即可间接实现丁酰胆碱酯酶的定量测定。在本研究采用的基于α-环糊精膜的氯化丁酰胆碱离子选择性电极检测器的顺序注射分析体系中，当氯化丁酰胆碱浓度为2.0 mmol·dm⁻³、反应时间为5.0 min时，可对浓度范围为0.015~0.50 units/mL的丁酰胆碱酯酶进行定量检测。该方法对丁酰胆碱酯酶的检出限为4.8×10⁻³ units/mL。