ChIP-Seq of H3K27ac in Wild Type (WT), and Actb-/- (KO) Mouse Embryonic Fibroblasts

Genome-wide ChIP-Seq of H3K27ac in WT and ß-actin KO MEFs to assess the role of nulcear actin in regulating histone acetylation Overall design: ChIP-Seq was performed for 6 samples (3 replicates of KO cells, 2 replicates of WT cells and one input control)

本数据集针对野生型（Wild Type, WT）与β-肌动蛋白敲除（Knock Out, KO）小鼠胚胎成纤维细胞（Mouse Embryo Fibroblasts, MEFs）开展全基因组范围内的组蛋白H3赖氨酸27乙酰化（Histone H3 Lysine 27 Acetylation, H3K27ac）染色质免疫共沉淀测序（ChIP-Seq）实验，以探究核肌动蛋白在调控组蛋白乙酰化过程中的作用。实验整体设计：本次共完成6个样本的ChIP-Seq测序，包含3组敲除细胞生物学重复、2组野生型细胞生物学重复以及1个输入对照样本。