Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool26_h1930002_Pro

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Apply snmC-seq3 to brain regions to identify epigenomic landcape of the adult human brains

阐明人类大脑的细胞架构，对于理解其多样功能与复杂动物行为至关重要。本研究对人类大脑多个脑区实施单细胞核甲基化测序（single nucleus methylation sequencing），成功鉴定出不同的细胞簇并构建了层级分类系统。这些细胞簇既包含已被认知的主要脑细胞类型，也涵盖潜在的细胞亚型。本研究利用上述数据解析了各细胞簇的表观基因组特征，并为每一类细胞簇定义了特异性调控元件。此外，本研究将snmC-seq3技术应用于脑区样本，以解析成年人类大脑的表观基因组图谱。