Data from: Genome sequencing of a single tardigrade Hypsibius dujardini individual

Tardigrades are ubiquitous microscopic animals that play an important role in the study of metazoan phylogeny. Most terrestrial tardigrades can withstand extreme environments by entering an ametabolic desiccated state termed anhydrobiosis. Due to their small size and the non-axenic nature of laboratory cultures, molecular studies of tardigrades are prone to contamination. To minimize the possibility of microbial contaminations and to obtain high-quality genomic information, we have developed an ultra-low input library sequencing protocol to enable the genome sequencing of a single tardigrade Hypsibius dujardini individual. Here, we describe the details of our sequencing data and the ultra-low input library preparation methodologies.

缓步动物（Tardigrades）是一类广泛分布的微型动物，在后生动物（Metazoa）系统发育研究中具有重要地位。多数陆生缓步动物可通过进入代谢停滞的干燥状态——即失水隐生（anhydrobiosis）——来耐受极端环境。鉴于缓步动物体型微小，且其实验室培养体系为非无菌（non-axenic）状态，相关分子研究极易受到微生物污染。为降低微生物污染风险并获取高质量基因组信息，本研究开发了一套超低起始量建库测序流程（ultra-low input library sequencing protocol），可实现单个杜氏高生熊虫（Hypsibius dujardini）个体的基因组测序。本文详细阐述了本研究的测序数据及超低起始量建库方法的具体细节。