Characteristics of Suppression Subtractive Hybridization sequences

A. caninum sequences identified by Suppression Subtractive Hybridization (SSH) were considered to be new if they shared less than 95% identity and at least 30 base pairs of overlap with any of the publicly available A. caninum expressed sequence tags. SSH sequences were considered to be unique to A. caninum if a similar sequence could not be identified from another species (E-value cut-off, 1��10?6) in dbEST, GenBank, or Swissprot.通过抑制性消减杂交（Suppression Subtractive Hybridization，SSH）鉴定得到的犬钩虫（A. caninum）序列，若与任一公开可用的犬钩虫表达序列标签（expressed sequence tags，EST）的序列一致性低于95%，且重叠区域至少包含30个碱基对，则被视为新序列。若此类序列在dbEST、GenBank或Swiss-Prot数据库中无法从其他物种中检索到相似序列（E值阈值为1×10⁻⁶），则被认定为犬钩虫（A. caninum）所特有的序列。