Gene expression signature of Gata6 positive epidermal cells.

To characterize gene expression in Gata6 positive epidermal cells we analyzed a Gata6 reporter mouse in which the endogenous Gata6 promoter drives expression of mTomato. We performed flow cytometry followed by transcriptome analysis. We compared four subpopulations of telogen epidermal cells: Gata6+/Itga6+ cells, Gata6+/itga6- cells, CD34+/Itga6+ cells (which are Gata6-) and all remaining Itga6+ cells (Gata6-/CD34-). The RNA was isolated from age and sex matched mice. We compared four subpopulations of telogen epidermal cells: Gata6+/Itga6+ cells, Gata6+/itga6- cells, CD34+/Itga6+ cells (which are Gata6-) and all remaining Itga6+ cells (Gata6-/CD34-). The RNA was isolated from age and sex matched mice. Three biological replicates for each cell population were analyzed

为了表征Gata6阳性表皮细胞的基因表达特征，我们分析了一株Gata6报告基因小鼠，该小鼠的内源性Gata6启动子可驱动mTomato的表达。我们先开展流式细胞术分析，随后进行转录组分析。我们比较了休止期表皮细胞的四个亚群：Gata6+/Itga6+细胞、Gata6+/Itga6-细胞、CD34+/Itga6+细胞（该亚群为Gata6阴性）以及其余所有Itga6+细胞（Gata6-/CD34-）。实验所用RNA均提取自年龄与性别相匹配的小鼠。我们比较了休止期表皮细胞的四个亚群：Gata6+/Itga6+细胞、Gata6+/Itga6-细胞、CD34+/Itga6+细胞（该亚群为Gata6阴性）以及其余所有Itga6+细胞（Gata6-/CD34-）。实验所用RNA均提取自年龄与性别相匹配的小鼠。每个细胞群体均设置三次生物学重复用于后续分析。