The sample includes RAD-seq data from one Vanessa cardui female and 95 offspring, for construction of a linkage map. The DNA was extracted with a modified saltextraction method and digested with ecoR1
Although numerous linkage maps have been constructed in the genus Populus, they are typically sparse and thus have limited applications due to low throughput of traditional molecular markers. Restrict
A mapping population of 167 BC1 progeny was developed from the cross ('WF1680' x '1588') x 'WF1680'. The plants were genotyped with RFLP markers using Trifolium pratense cDNA probes.
The linkage maps derived from four populations were integrated. One population is 95 F2 progeny of the cross 'Io' x 'F2'. One population is 145 F5:6 recombinant inbred lines from the cross 'Io' x 'F2'