Crystallographic projects at Structural Biology Core Facility, JU - bringing the state of art support to the users in need.

Structural biology is very informative discipline providing strong support to virtually any biochemistry related project, however in many cases a lack of expertise is a barrier limiting application of structural biology techniques in the most exciting projects. Foundation of Structural Biology Core Facility (SBCF) at Jagiellonian University (JU, Krakow, Poland) was motivated by a need of providing a support of local experts to biochemists who could potentially benefit from detailed knowledge of their proteins of interest. SBCF is based on two pillars - cryo-EM and macromolecular crystallography. The later being conducted with the use of state-of-art beamlines at European synchrotron radiation sources. Since 2019 SBCF assisted in over 30 peer-reviewed publications. With this proposal we'd like to apply for an access to state of the art crystallographic bemlines at ESRF to help our user community to benefit from the brightest and most advanced X-ray sources in Europe.

结构生物学是一门信息详实的学科，可为几乎所有生物化学相关研究项目提供强有力的支撑；然而在诸多场景中，专业技术专长的缺失往往成为限制前沿项目应用结构生物学技术的瓶颈。波兰克拉科夫雅盖隆大学（Jagiellonian University, JU）结构生物学核心设施（Structural Biology Core Facility, SBCF）的设立，旨在为生物化学家配备本地结构生物学家的专业支持，帮助他们借助对目标蛋白的深度解析获取研究收益。SBCF以两大技术支柱为核心——冷冻电镜（cryo-EM）与大分子晶体学，其中大分子晶体学依托欧洲同步辐射光源的顶尖光束线开展实验。自2019年以来，SBCF已协助完成30余篇经同行评议的学术论文。本提案旨在申请使用欧洲同步辐射装置（ESRF）的顶尖晶体学光束线，助力我们的用户群体借助欧洲性能最优、最为先进的X射线光源开展研究。