Single cell RNA sequencing of In vivo mouse xenograft tissues from two human TRa1 anaplastic thyroid cancer cell line

We used scRNA-seq technology to study mouse xenograft tissues from two human TRa1 anaplastic thyroid cancer (ATC) cell lines. Our analysis demonstrated that TRa1 functions as a transcription factor through multiple signaling pathways to suppress tumor growth. Importantly, scRNA-seq analysis showed that TRa1-induced PAX8, via its transcription program, shifts the cell landscape of ATC toward a differentiated state. ATC cells isolated from human tumors (11T and 16T) were subcutaneously inoculated into mice to grow into xenograft tumors. Single cells were dissociated from the tumors of the 8-week mice and then analyzed by single-cell RNA sequencing (scRNA-seq).

本研究采用单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）技术，对源自两株人源TRa1间变性甲状腺癌（anaplastic thyroid cancer, ATC）细胞系的小鼠异种移植组织进行分析。研究结果显示，TRa1可作为转录因子，通过多条信号通路抑制肿瘤生长。尤为重要的是，单细胞RNA测序分析表明，TRa1可通过自身转录调控程序诱导PAX8表达，将间变性甲状腺癌的细胞状态谱向分化成熟状态重塑。本研究将从人源肿瘤（11T与16T）中分离得到的ATC细胞经皮下接种植入小鼠体内，使其形成异种移植肿瘤；随后从接种8周的小鼠肿瘤组织中解离获取单细胞，再通过单细胞RNA测序技术完成相关分析。