Characterizing the Key Metabolic Pathways of the Neonatal Mouse Heart Using a Quantitative Combinatorial Omics Approach

The heart of new-born mouse has exceptional capacity to regenerate from myocardial injury. However, the mouse heart loses this capacity within the first week of its life. In order to elucidate the molecular mechanisms taking place in the mouse heart during the loss of its regenerative potential, a systematic comparison of 1-day and 7-day-old mouse hearts was performed at the proteomic, metabolomic, and transcriptomic levels. Overall design: RNA-seq gene expression of hearts of postnatal day 1 and day 7 Hsd:ICR(CD-1®) male mice新生小鼠的心脏具备卓越的心肌损伤再生能力，但该能力会在小鼠出生后的第一周内丧失。为阐明小鼠心脏丧失再生潜能过程中的分子机制，研究人员在蛋白质组学（proteomics）、代谢组学（metabolomics）与转录组学（transcriptomics）层面，对出生后1天和7天的小鼠心脏开展了系统性比较分析。整体实验设计：对出生后第1天及第7天的Hsd:ICR(CD-1®)雄性小鼠心脏进行RNA测序（RNA-seq）基因表达检测。