Genome-wide maps of chromatin state in MDA-MB-231 cells

In order to figure out the genomic binding landscape of CARM1, we analyzed the genome-wide transcriptional targets for CARM1 using the Chromatin immunoprecipitation based-deep sequencing (ChIP-seq) in MDA-MB-231 cells with antibodies against CARM1. Using Illumina instrument, we found lots of CARM1-specific binding peaks and strong enrichment on the promoters of selected genes involved in classical pathways. This study gave us a new understanding of the role of CARM1 in chromatin status and gene transcription. Examination of CARM1 target genes in MDA-MB-231 cell line

为解析CARM1的全基因组结合图谱，本研究采用抗CARM1抗体，在MDA-MB-231细胞中通过基于染色质免疫共沉淀的深度测序（Chromatin immunoprecipitation based-deep sequencing，ChIP-seq）技术分析CARM1的全基因组转录靶标。借助Illumina测序平台完成测序后，我们检测到大量CARM1特异性结合峰，且在经典通路相关候选基因的启动子区域呈现显著富集。本研究为理解CARM1在染色质状态调控与基因转录中的功能提供了新的认知。本数据集聚焦MDA-MB-231细胞系中CARM1靶基因的检测分析。