Phosphoproteome dynamics upon changes in plant water status reveal early events associated with rapid growth adjustment in maize leaves.

PRIDE ID: 25003. Data published as part of Mol Cell Proteomics. 2012 Jul 10 . From the Abstract: {{i}}Aiming at a better understanding of early responses to contrasting plant water statuses, we carried out a survey of the protein phosphorylation events in the growing zone of maize leaves upon a range of water regimes. In this study, the impact of mild and severe water deficits were evaluated in comparison with constant optimal watering and with recovery periods lasting 5, 10, 20, 30, 45 and 60 min. Using four biological replicates per treatment and a robust quantitative phosphoproteomic methodology based on stable-isotope labeling, we identified 3664 unique phosphorylation sites on 2496 proteins.{{/i}}

PRIDE编号：25003。本数据发表于《分子与细胞蛋白质组学》（Molecular & Cellular Proteomics），2012年7月10日。摘要内容如下：{{i}}为深入解析植物在不同水分状态下的早期响应机制，本研究针对不同水分条件下玉米叶片生长区的蛋白质磷酸化事件开展了系统性调查。本研究以持续最优供水为对照，设置轻度、重度水分胁迫处理，并设置时长分别为5、10、20、30、45、60分钟的复水组，以此评估不同程度水分亏缺的影响。本研究每个处理设置4次生物学重复，并采用基于稳定同位素标记（stable-isotope labeling）的稳健定量磷酸蛋白质组学方法，最终在2496个蛋白质上鉴定到3664个独特的磷酸化位点。{{/i}}